Part:BBa_K4370012

sgRNA_gpm_STREPTO

This part encodes a single guide RNA targeting the gpm gene encoding a 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (short name: phosphoglyceromutase) in Streptomyces. It has been designed to target different strains such as S. ambofaciens ATCC 23877, S. bottropensis ATCC 25435, and S. venezuelae ATCC 10712.

Usage and Biology

A decrease of gpm expression has been shown as required to allow the implementation of the Calcin cycle in E. coli (Antonovsky et al., Cell, 2016). This part could therefore be useful to implement a CO₂ fixation module in Streptomyces.

We have engineered genetic tools via the Crispr-dCas9 system to switch off gpm and force the appearance of the Calvin cycle in their primary metabolism.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Antonovsky N, Gleizer S, Noor E, Zohar Y, Herz E, Barenholz U, Zelcbuch L, Amram S, Wides A, Tepper N, Davidi D, Bar-On Y, Bareia T, Wernick DG, Shani I, Malitsky S, Jona G, Bar-Even A, Milo R. "Sugar Synthesis from CO₂ in Escherichia coli." Cell. 2016 Jun 30;166(1):115-25

https://doi.org/10.1016/j.cell.2016.05.064